منابع مشابه
Poxvirus DNA primase.
Poxviruses are large enveloped viruses that replicate in the cytoplasm of vertebrate or invertebrate cells. At least six virus-encoded proteins are required for synthesis and processing of the double-stranded DNA genome of vaccinia virus, the prototype member of the family. One of these proteins, D5, is an NTPase that contains an N-terminal archaeoeukaryotic primase domain and a C-terminal supe...
متن کاملPoxvirus DNA Replication
Poxviruses, of which vaccinia is the prototype, are complex DNA viruses that display an unusual degree of physical and genetic autonomy from the host cell. The vaccinia genome encodes most, if not all, of the functions required for three temporally regulated phases of transcription and for DNA replication (Moss 1990a,b, 1994; Traktman 1990a,b, 1991). In addition, the 192-kb genome encodes numer...
متن کاملPoxvirus DNA replication.
Poxviruses are large, enveloped viruses that replicate in the cytoplasm and encode proteins for DNA replication and gene expression. Hairpin ends link the two strands of the linear, double-stranded DNA genome. Viral proteins involved in DNA synthesis include a 117-kDa polymerase, a helicase-primase, a uracil DNA glycosylase, a processivity factor, a single-stranded DNA-binding protein, a protei...
متن کاملPolypeptide structure of DNA primase from a yeast DNA polymerase-primase complex.
An immunoaffinity chromatographic procedure was developed to purify DNA polymerase-DNA primase complex from crude soluble extracts of yeast cells. The immunoabsorbent column is made of mouse monoclonal antibody to yeast DNA polymerase I covalently linked to Protein A-Sepharose. Purification of the complex involves binding of the complex to the immunoabsorbent column and elution with concentrate...
متن کاملYeast DNA Primase Genes Affect Different Aspects of DNA Metabolism and Interactions in the DNA Polymerase a-Primase Complex
Different pril and pri2 conditional mutants of Saccharomyces cereuisiae altered, respectively, in the small (p48) and large (p58) subunits of DNA primase, show an enhanced rate of both mitotic intrachromosomal recombination and spontaneous mutation, to an extent which is correlated with the severity of their defects in cell growth and DNA synthesis. These effects might be attributable to the fo...
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ژورنال
عنوان ژورنال: Proceedings of the National Academy of Sciences
سال: 2007
ISSN: 0027-8424,1091-6490
DOI: 10.1073/pnas.0709276104